After the blood-stained research expedition in Anacondas: The Hunt for the Blood Orchid (2004), Peter Murdoch of Wexel Hall Pharmaceuticals continues to play with fire, and as a result, a pair of aggressive giant serpents escapes. Now, to capture the super-reptiles, Murdoch enlists the help of the experienced animal hunter, Hammett, who, along with Amanda Hayes, the herpetologist responsible for the genetically enhanced snakes, risk life and limb to track them down. But, this time, Queen Anaconda is pregnant. Is there an escape from the new breed of slithering terror?