The merry family of the rare Blue Spix Macaws, Blu and Jewel, along with their three adorable chicks, now enjoy an easy existence in the sun-kissed city of Rio. However, with the kids becoming more and more like humans, Linda and Tulio's unexpected discovery of an entire colony of feathery relatives somewhere deep into the impenetrable Amazon rainforest will have the family back to the jungle. But an old enemy waits there; and to make matters worse, apart from a difficult father-in-law, a rapacious group of illegal woodcutters threaten to put in jeopardy not only the region but also all life forms in the habitat. Can Jewel and Blu thwart their plans, and find their roots in the process?